Diego Llorente is set to return to Spain's training camp on Friday after returning a negative test for coronavirus.
The Royal Spanish Football Federation (RFEF) said the Leeds United defender had become the second player in camp to test positive for COVID-19 ahead of their Euro 2020 opener, with captain Sergio Busquets the first to have done so.
Llorente was removed from the team's base and was due to begin 10 days of self-isolation in line with COVID-19 health protocols.
However, a statement from the RFEF said a follow-up test undertaken on Wednesday came back negative, and that there are indications of a "false positive" from the original test.
Llorente will undergo further tests on Thursday and Friday and will be allowed to return to camp if both come back negative.
Busquets' positive result plunged Spain's preparations for their Group E opener against Sweden on Monday into chaos, with group training suspended and all members of the squad preparing individually.
It meant Spain's U-21 squad was drafted in for Tuesday's 4-0 win over Lithuania, a match that was supposed to be the main team's final warm-up for the rearranged tournament.
A 17-man parallel squad remains in place at Spain's base should any further replacements be needed.